Processing time and output
Different processing timing is necessary for different performances, as indicated below:
| Activities | Processing time |
| Protein characterization | |
| Protein and peptide analysis by HPLC/MS and MS/MS | 3 days |
| Sample purification (zip tip or similar) and intact protein MW determination by MALDI or nanospray off line MS | 3 days |
Protein identification: PMF and sequencing | |
| PTM-definition (type and site) by mass spectrometry | 2 weeks |
| PTM-characterization: validation by Western Blotting and 2DE +/- removal of the PTM (glycosylation, phosphorylation) | 2 weeks |
| Identification of a spot or band by in gel digestion and PMF | 1 week |
| Identification of a spot or band by in gel digestion and LC-MS/MS | 2 weeks |
| Biomarker profiling in biological samples by 2DE, image analysis and statistical evaluation | |
| Differential protein display in control vs patient biological samples: | |
| plasma | |
| serum | |
| urine | |
| tissue |
In case of maintenance or out of order instrumentation as well as when multiple samples are submitted, the processing time can be longer.
Output of the results will be sent in an Excel or Word format, along with information on: mass spectrometer settings, protein database version, search parameters, experimental conditions for 2DE, constraints for image analysis and statistical evaluation.
When our Facility will send the results of the analysis (by fax or e-mail) then the user will send the order of payment both to the Grant Office and to our Facility. The Grant Office will give the invoice back.
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